Format Conversion
-Combine FASTA
-EMBL to FASTA
-EMBL Feature Extractor
-EMBL Trans Extractor
-Filter DNA
-Filter Protein
-GenBank to FASTA
-GenBank Feature Extractor
-GenBank Trans Extractor
-One to Three
-Range Extractor DNA
-Range Extractor Protein
-Reverse Complement
-Split Codons
-Split FASTA
-Three to One
-Window Extractor DNA
-Window Extractor Protein
Sequence Analysis
-Codon Plot
-Codon Usage
-CpG Islands
-DNA Molecular Weight
-DNA Pattern Find
-DNA Stats
-Fuzzy Search DNA
-Fuzzy Search Protein
-Ident and Sim
-Multi Rev Trans
-Mutate for Digest
-ORF Finder
-Pairwise Align Codons
-Pairwise Align DNA
-Pairwise Align Protein
-PCR Primer Stats
-PCR Products
-Protein GRAVY
-Protein Isoelectric Point
-Protein Molecular Weight
-Protein Pattern Find
-Protein Stats
-Restriction Digest
-Restriction Summary
-Reverse Translate
-Translate
Sequence Figures
-Color Align Conservation
-Color Align Properties
-Group DNA
-Group Protein
-Primer Map
-Restriction Map
-Translation Map
Random Sequences
-Mutate DNA
-Mutate Protein
-Random Coding DNA
-Random DNA Sequence
-Random DNA Regions
-Random Protein Sequence
-Random Protein Regions
-Sample DNA
-Sample Protein
-Shuffle DNA
-Shuffle Protein
Miscellaneous
-Home
-IUPAC codes
-Genetic codes
-Browser compatibility
-Mirror this site
-Use this site off-line
-About this site
-Acknowledgments
-Reference
The Sequence Manipulation Suite Copyright © 2000, 2004 Paul Stothard. Send questions and comments to
[email protected]
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Sequence Manipulation Suite:
Protein Isoelectric Point
Protein Isoelectric Point calculates the theoretical pI (isoelectric point) for the protein sequence you enter. Use Protein Isoelectric Point when you want to know approximately where on a 2-D gel a particular protein will be found.
Paste the raw sequence or one or more FASTA sequences into the text area below. Input limit is 200,000,000 characters.
>sequence 1 GAMPSTRV >sequence 2 MPSTYLLQ
Add
1
2
3
4
5
copies of
Nothing
AU1 (DTYRYI)
AU5 (TDFYLK)
BTag (QYPALT)
c-myc (EQKLISEEDL)
FLAG (DYKDDDDK)
GFP (MSKGEELFTG...)
Glu-Glu (EYMPME)
Glu-Glu (EFMPME)
GST (MSPILGYWKI...)
HA (YPYDVPDYA)
His6 (HHHHHH)
HSV (QPELAPEDPE...)
HTTPHH (HTTPHH)
IRS (RYIRS)
KT3 (PPEPET)
Protein C (EDQVDPRLID...)
S-Tag (KETAAAKFER...)
SBP (MDEKTTGWRG...)
T7 (MASMTGGQQM...)
V5 (GKPIPNPLLG...)
VSV-G (MNRLGK)
to each sequence.
Use pK values from
EMBOSS
DTASelect
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